Name | Bromocresol Green |
Synonyms | BCG BROMCRESOL GREEN BROMOCRESOL BLUE Bromcresol Green vertdebromocresol Bromocresol Green bromcresol green spray BCG, Mycobacterium bovis Bromocresol green solution bromcresol green crystalline Bromocresol Green Sultone Form BROMOCRESOL GREEN SULTONE FORM BACILLUS OF CALMETTE AND GUERIN tetrabromo-m-cresolphthaleinsulfone tetrabromo-m-cresolsulphonephthalein 3,3,5,5-Tetrabromo-m-cresolsulfonephthalein bromocresol green for microscopy and complexometry Phenol,4,4'-(3H-2,1-benzoxathiol-3-ylidene)bis(2,6-dibromo-3-methyl-,S,S-dioxide |
CAS | 76-60-8 |
EINECS | 200-972-8 |
InChI | InChI=1/C21H14Br4O5S/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21/h3-8,26-27H,1-2H3 |
Molecular Formula | C21H14Br4O5S |
Molar Mass | 698.01 |
Density | 0.79g/mLat 20°C |
Melting Point | 225 °C (dec.) (lit.) |
Boling Point | 626.0±55.0 °C(Predicted) |
Flash Point | 12°C |
Water Solubility | slightly soluble |
Solubility | Soluble in ethanol, ether and ethyl acetate, soluble in benzene, slightly soluble in water |
Appearance | White or bright yellow crystals |
Color | Pale orange |
Maximum wavelength(λmax) | ['423nm, 444nm, 617nm'] |
Merck | 14,1386 |
BRN | 372527 |
pKa | 4.7(at 25℃) |
PH | 3.8~5.4 |
Storage Condition | no restrictions. |
Stability | Stable. Incompatible with strong oxidizing agents. |
Sensitive | Easily absorbing moisture |
MDL | MFCD00005874 |
Use | Used as acid-base indicator |
Risk Codes | R11 - Highly Flammable R36 - Irritating to the eyes R67 - Vapors may cause drowsiness and dizziness R36/37/38 - Irritating to eyes, respiratory system and skin. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
Safety Description | S7 - Keep container tightly closed. S16 - Keep away from sources of ignition. S24/25 - Avoid contact with skin and eyes. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S37/39 - Wear suitable gloves and eye/face protection S36 - Wear suitable protective clothing. |
UN IDs | UN 1219 3/PG 2 |
WGK Germany | 3 |
RTECS | SJ7456000 |
TSCA | Yes |
HS Code | 29349990 |
Hazard Class | 3 |
Packing Group | II |
Reference Show more | 1. Fu Junjing, Li Chunfeng. Efficacy of tigecycline in the treatment of multiple or pan-drug resistant Acinetobacter baumannii Pneumonia [J]. Journal of Xinxiang Medical University, 2016(6th issue):504-507. 2. Liu Huilin, Wang Yuzhen, Yu Xinyu, etc. Effects of highland barley whole grain and wheat bran on the quality of biscuits [J]. Science and Technology of food industry, 2019, 40(09):62-67. 3. Li Tianyu, Zhang Songshan, Zhang Zhisheng, Zhang Shou, Lei Yuanhua, Xie Peng, Bai Yueyu, Zhang Yang, Sun Baozhong. Variation of edible quality and collagen structure of three kinds of bovine skin during heating [J]. Meat Research, 2020,34(12):18-23. |
pH range of acid-base indicator discoloration | 3.8(YELLOW)-5.4(BLUE) |
Main application | Sol-gel matrix, fuel cells, sensors, display device, inks, paints, one-steptester for ammonia, cosmet-tester for ammonia, packaging system, vaginal infection test method, drugs, dental materials |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
Overview | bromocresol green is also known as 3,3 ', 5,5'-tetrabromo-m-cresol sulfonyl phthalate, gamma-sultone. The precipitates from acetic acid were fine, pale yellow crystals. Relative molecular mass 698.05. The melting point was 218-219 °c. Slightly soluble in water, soluble in ethanol, ethyl ether, ethyl acetate and benzene. Very sensitive to alkali, alkaline aqueous solution was a special blue-green. Bromocresol green can be used as an indicator, which is yellow at pH = 3.8 and blue-green at pH = 5.4. |
Application | bromocresol green (BCG), as an important stain, has been widely used in analytical field. 1)CN201110395918.7 discloses a method for testing the content of free amines in NMP. The present invention uses an acid-base titration method to test the content of amines in NMP by preparing a bromocresol green-methyl red indicator. 2)CN201811547295.9 discloses a mixed indicator test paper for Measuring ammonia concentration in air and a preparation and detection method thereof, belonging to the technical field of environmental monitoring and industrial hygiene. |
preparation | A preparation method of bromocresol green, the steps are as follows:(1) 1.5 of bromine was dissolved in 1.25 L of glacial acetic acid solution to obtain bromine-glacial acetic acid mixture;(2) 2kg of pure m-cresol Violet was dissolved in 2.5 L of glacial acetic acid;(3) the bromine-glacial acetic acid mixture obtained in step (1) is slowly dropped into the solution in step (2) at a temperature not exceeding 80 ° C. After addition, stirring is continued and the mixture is maintained at 80-90 ° C. For 24 hours, after cooling, filtering, precipitation with glacial acetic acid wash two times, air drying or suction, drying at 50~60 deg C to obtain crude bromocresol green. In addition, the crude bromocresol green is recrystallized from benzene or glacial acetic acid to obtain pure bromocresol green, which is white in color and has a melting point of 218-9 °c. |
Use | cell stain used as acid-base indicator acid-base indicator, pH color change range 3.8 (yellow) ~ 5.4 (blue-green) acid-base indicator. The sodium salt is often used for the Colorimetric Determination of pH. Acid-base indicator, pH 3.8 (yellow) ~ 5.4 (blue). Its sodium salt aqueous solution is used as a color developing agent for Spectrophotometric Determination of pH value. It is used as a reagent for the determination of aliphatic hydroxy acids and alkaloids by thin layer chromatography and an extraction and separation agent for the determination of quaternary ammonium cations by spectrophotometry. |
autoignition temperature | 399°C |